Composite

Part:BBa_K5403012:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-01)


Fusion protein for OmpAN21-based membrane anchoring of GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 505
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A flexible linker with the sequence GSGSGSGSAS was used to fuse the membrane anchoring domain and GFP. The flexibility prevents the individual domains from misfolding as a result of being fused to one another.


Source

E.coli (OmpA) and Aequorea victoria (GFP).

References